Coding

Part:BBa_K112114:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


a~Ligase!


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1312
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1312
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 336


Design Notes

Source

PCR mv024/mv014 on pBca1256-K112109    (1499bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112114
-----------------------------------------------
mv024	Forward Biobricking of a~ligase!
ccataGAATTCatgAGATCTaacaatgattcttaaaattctgaacg
mv014	Reverse Biobricking of ligase!
cgttaGGATCCtcatagaccagttacctcatg

References